Identification of Setaria cervi heat shock protein 70 by mass spectrometry and its evaluation as diagnostic marker for lymphatic filariasis.
Identifieur interne : 005855 ( Main/Exploration ); précédent : 005854; suivant : 005856Identification of Setaria cervi heat shock protein 70 by mass spectrometry and its evaluation as diagnostic marker for lymphatic filariasis.
Auteurs : Saurabha Srivastava [Inde] ; Elesela Srikanth ; Eva Liebau ; Sushma RathaurSource :
- Vaccine [ 1873-2518 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (immunologie), Bovins, Femelle, Filariose lymphatique (diagnostic), Filariose lymphatique (génétique), Filariose lymphatique (immunologie), Humains, Immunoglobuline G (immunologie), Marqueurs biologiques, Mâle, Peptides (génétique), Peptides (immunologie), Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Protéines du choc thermique HSP70 (génétique), Protéines du choc thermique HSP70 (immunologie), Réactions croisées (génétique), Réactions croisées (immunologie), Setaria (nématode) (génétique), Setaria (nématode) (immunologie), Similitude de séquences d'acides aminés, Spectrométrie de masse, Séquence d'acides aminés (génétique), Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : Filariose lymphatique, Peptides, Protéines d'helminthes, Protéines du choc thermique HSP70, Réactions croisées, Setaria (nématode), Séquence d'acides aminés, Wuchereria bancrofti.
- immunologie : Anticorps antihelminthe, Filariose lymphatique, Immunoglobuline G, Peptides, Protéines d'helminthes, Protéines du choc thermique HSP70, Réactions croisées, Setaria (nématode), Wuchereria bancrofti.
- Animaux, Bovins, Femelle, Humains, Marqueurs biologiques, Mâle, Similitude de séquences d'acides aminés, Spectrométrie de masse.
English descriptors
- KwdEn :
- Amino Acid Sequence (genetics), Animals, Antibodies, Helminth (immunology), Biomarkers, Cattle, Cross Reactions (genetics), Cross Reactions (immunology), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (genetics), Elephantiasis, Filarial (immunology), Female, HSP70 Heat-Shock Proteins (genetics), HSP70 Heat-Shock Proteins (immunology), Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Immunoglobulin G (immunology), Male, Mass Spectrometry, Peptides (genetics), Peptides (immunology), Sequence Homology, Amino Acid, Setaria Nematode (genetics), Setaria Nematode (immunology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
- MESH :
- chemical , genetics : HSP70 Heat-Shock Proteins, Helminth Proteins, Peptides.
- chemical , immunology : Antibodies, Helminth, HSP70 Heat-Shock Proteins, Helminth Proteins, Immunoglobulin G, Peptides.
- diagnosis : Elephantiasis, Filarial.
- genetics : Amino Acid Sequence, Cross Reactions, Elephantiasis, Filarial, Setaria Nematode, Wuchereria bancrofti.
- immunology : Cross Reactions, Elephantiasis, Filarial, Setaria Nematode, Wuchereria bancrofti.
- Animals, Biomarkers, Cattle, Female, Humans, Male, Mass Spectrometry, Sequence Homology, Amino Acid.
Abstract
Using mass spectrometry and immunological approaches, a heat shock protein 70 associated with lymphatic filariasis (LF) has been identified from a bovine filarial parasite Setaria cervi. A heat shock protein was detected in different life stages of S. cervi when exposed to an elevated temperature of 44 degrees C. A combination of ATP-agarose column chromatography and electro-elution was used for its purification from adult female extract. On closer examination, it migrated as a single band at 68 kDa on 10% SDS-PAGE. Peptide sequences TTPSYVAFTDTER, DSGAIAGLNVLR, IINEPTAAAIAYGLDK, NALESYAFNMK and LLSDFFSGK were obtained through MALDI-LC/MS analysis. Confirmation of peptides was accomplished by MASCOT database which showed substantial sequence homology with S. digitata, Wuchereria bancrofti, and Caenorhabditis elegans. Multiple sequence alignment using Clustal W showed 98% identity with W. bancrofti and only 28% with human HSP70. Furthermore, the antigenicity plot has shown that the highly antigenic amino acid residues are constituted within the conserved peptides. These observations suggest a plausible biological connection of ScHSP70 with the disease and its strong immunogenic nature. ScHSP70 showed antigenic cross-reactivity with IgG class of antibody in different categories of filarial sera. However, when IgG subclasses were tested, IgG4 showed high specificity and sensitivity with asymptomatic microfilaraemic sera.
DOI: 10.1016/j.vaccine.2009.06.044
PubMed: 19573640
Affiliations:
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d'acides aminés</term><term>Spectrométrie de masse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using mass spectrometry and immunological approaches, a heat shock protein 70 associated with lymphatic filariasis (LF) has been identified from a bovine filarial parasite Setaria cervi. A heat shock protein was detected in different life stages of S. cervi when exposed to an elevated temperature of 44 degrees C. A combination of ATP-agarose column chromatography and electro-elution was used for its purification from adult female extract. On closer examination, it migrated as a single band at 68 kDa on 10% SDS-PAGE. Peptide sequences TTPSYVAFTDTER, DSGAIAGLNVLR, IINEPTAAAIAYGLDK, NALESYAFNMK and LLSDFFSGK were obtained through MALDI-LC/MS analysis. Confirmation of peptides was accomplished by MASCOT database which showed substantial sequence homology with S. digitata, Wuchereria bancrofti, and Caenorhabditis elegans. Multiple sequence alignment using Clustal W showed 98% identity with W. bancrofti and only 28% with human HSP70. Furthermore, the antigenicity plot has shown that the highly antigenic amino acid residues are constituted within the conserved peptides. These observations suggest a plausible biological connection of ScHSP70 with the disease and its strong immunogenic nature. ScHSP70 showed antigenic cross-reactivity with IgG class of antibody in different categories of filarial sera. However, when IgG subclasses were tested, IgG4 showed high specificity and sensitivity with asymptomatic microfilaraemic sera.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Liebau, Eva" sort="Liebau, Eva" uniqKey="Liebau E" first="Eva" last="Liebau">Eva Liebau</name><name sortKey="Rathaur, Sushma" sort="Rathaur, Sushma" uniqKey="Rathaur S" first="Sushma" last="Rathaur">Sushma Rathaur</name><name sortKey="Srikanth, Elesela" sort="Srikanth, Elesela" uniqKey="Srikanth E" first="Elesela" last="Srikanth">Elesela Srikanth</name></noCountry><country name="Inde"><noRegion><name sortKey="Srivastava, Saurabha" sort="Srivastava, Saurabha" uniqKey="Srivastava S" first="Saurabha" last="Srivastava">Saurabha Srivastava</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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